On-line repository for Hypocrea/Trichoderma DNA sequences for correct taxa identification verified using ARB technology and phylogenetic analysis

01.12.2004 - 31.03.2007
Research funding project
The first version of the genus-specific fungal oligonucleotide BarCode for the identification of Hypocrea and Trichoderma species ¿ TrichOKey v. 1, was developed in 2005. The program provides an on-line method for the quick molecular identification of an unknown isolate at the genus, clade and species levels based on a diagnostic combination of several oligonucleotides (hallmarks) specifically nested within the internal transcribed spacer 1 and 2 (ITS1 and 2) sequences of the rRNA gene cluster. In this manuscript we report on the newly integrated modules to increase the reliability of the result and present the second version of the program - TrichOKey v. 2, which is powered by simultaneous identification of multiple sequences, complementary similarity search tool and has an advanced graphic interface.

People

Project leader

Project personnel

Institute

Grant funds

  • Hochschuljubiläumsfonds der Stadt Wien (National) Anniversary Fund for Higher Education of the City of Vienna

Research focus

  • Beyond TUW-research focus: 100%

Keywords

GermanEnglish
Barcodebarcode
TrichodermaTrichoderma
Bioinformatikbioinformatics

Publications