Does Genomic Imprinting Affect Hydrolase Expression in Trichoderma reesei?

01.02.2012 - 31.07.2016
Research funding project
Strain degradation is an often-observed phenomenon in eukaryotic microorganisms and spans from the loss of pathogenicity to the degeneration of production strains used in biotechnology. Nonetheless such phenomenon hampers various basic research approaches as well as biotechnological applications, it is only poorly understood. One possible explanation for such phenomenon could be genetic imprinting. In the proposed study it is intended to investigate epigenetic effects on the expression of two closely related regulons (cellulase and xylanase) in the industrially important ascomycete Trichoderma reesei. The study will focus on strain- and growth condition-dependent differences in expression of both regulons. It will include wild type, production, and degenerated production strains as well as inducing and repressing conditions for both regulons. Enzymes resulting from the expression of both regulons are key players in the second-generation biofuel production. Both during strain improvement attempts as well as in biofuel production processes strain degeneration can be observed. The project will aid in a better understanding of such degeneration processes consequently leading to avoiding strategies.

People

Project leader

Institute

Grant funds

  • FWF - Österr. Wissenschaftsfonds (National) Richter Program Austrian Science Fund (FWF)

Research focus

  • Sustainable Production and Technologies: 100%

Keywords

GermanEnglish
LignozelluloseabbauLignocellulose degradation
ChromatinremodelierungChromatin remodelling
genomische PrägungGenomic imprinting
Zellulase- und XylanaseregulonCellulase and xylanase regulon
Trichoderma reeseiTrichoderma reesei

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