The project is about a new method for tissue clarification and staining. Tissue clarification refers to a chemical process that makes biological tissue transparent, much like a drop of oil on a white sheet of paper that makes it transparent at that particular point.

Tissue clarification found its way into biological research and analysis about 15 years ago.

The project is about a new method for tissue clarification and staining. Tissue clarification means that biological tissue is made transparent using a chemical process, similar to how a drop of oil on a white sheet of paper makes it transparent at this point.

Tissue clarification entered the field of biological research about 15 years ago. Initially, it was used to clarify mouse brains for studying nerve cells and their processes in their spatial arrangement.

 In the meantime, human tissues, such as surgical specimens from cancer operations, are used to investigate if the tumor has been completely removed or not. In general, all of these studies require strictly that the cells of interest have to be stained beforehand.

Since all chemicals must first penetrate the tissue, these 3D approaches are generally laborious and require a lot of time.

This naturally limits their practicality, especially in the clinic. We want to reduce this time by using high-frequency ultrasound, ie, all reactions take place in vessels that can be set into microscopic oscillations by ultrasound in the megahertz range.

In collaboration with the PCT company, we want to develop a device for biological-medical research, for fast clarification and staining of biological tissue utilizing high-frequency ultrasound with possible further use in the clinic.