164.355 Proteomics
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2020W, VO, 1.3h, 2.0EC


  • Semester hours: 1.3
  • Credits: 2.0
  • Type: VO Lecture
  • Format: Online

Learning outcomes

After successful completion of the course, students are able to identify proteomic questions in basic and applied research and quality control. The learning goal is to know different proteomic strategies and choose the appropriate strategy for diverse questions. Students will be able to design different proteomic experiments and theoretically apply sample preparation, proteomic analyses and bioinformatic analyses. This includes knowledge of protein and peptide separation techniques, such as electrophoresis and liquid chromatography (LC), (tandem) mass spectrometry (MS/MS), ion mobility (IMS) and LC-(IMS)-MS/MS of for identification, characterisation and quantification of proteins and peptides, and functional annotation of proteins and their modifications. Moreover, students will learn to apply statistical tools to proteomic data to calculate protein identification confidence and significant quantitative changes.

Subject of course

Proteomic strategies and methods: definition of research question, different sample preparation methods, identification, quantitation and annotation of proteins, peptides and proteomes, structural characterisation of proteins and their modifications, functional proteomics, high-through-put technologies, (tandem) mass spectrometry, ion mobility, liquid chromatography, bioinformatic tools

Teaching methods

Lecture with practical exercises (e.g. manual MS/MS spectrum interpretation of peptides, usage of webtools)

presentation and discussion of examples of proteomic applications

Mode of examination




Course dates

Wed16:00 - 19:0007.10.2020 - 25.11.2020 (LIVE)GoToMeeting Lecture
Proteomics - Single appointments
Wed07.10.202016:00 - 19:00 GoToMeeting Lecture
Wed14.10.202016:00 - 19:00 GoToMeeting Lecture
Wed28.10.202016:00 - 19:00 GoToMeeting Lecture
Wed04.11.202016:00 - 19:00 GoToMeeting Lecture
Wed11.11.202016:00 - 19:00 GoToMeeting Lecture
Wed25.11.202016:00 - 19:00 GoToMeeting Lecture

Examination modalities

Presentation and discussion of a proteomics application example

Course registration

Begin End Deregistration end
06.10.2020 14:00 31.10.2020 23:00 31.10.2020 23:00


Study CodeObligationSemesterPrecon.Info
066 490 Technical Chemistry Mandatory elective


A Tuwel course will be installed. Copies of the ppt-slides will be available via Tuwel/TISS.

Previous knowledge

Knowledge of protein chemistry, biochemistry and instrumental analytical chemistry is essential.