After successful completion of the course, students are able to understand advanced cell culture methods, are familiar with long term experiments, perform data collection and analysis, present experimental results/findings, and take part in scientific discussions.
1. Introduction to advanced culture and good cell culture practices (2h)
-review of aseptic techniques
-types of cells (primary, cell lines, iPSCs, stem cells with examples for use)
-culturing medium types (introduce differentiation concept)
-good cell culture practices (from the perspective of Grillari)
2. Introduction to 3D cell models (2h)
-what are the advantages of 3D models
- What are cellular changes between 2D and 3D culture
-What are spheroids and how to make them + examples of use
-What are organoids and how to make them + examples of use
3. Dynamic cell culture models (2h)
-Purpose of dynamic models, challenges and advantages
-Bioreactors- types, examples of use
-Microfluidics- types of chips and examples of use
4. Molecular biology analysis techniques in advanced cell culture (2h)
-PCR (explain different version, usage examples)
-FACS
-ELISA
-Western blot
-ECM analysis methods including histology
5. Microscopy techniques and analysis in advanced cell culture (2h)
-brightfield microscopy
-fluorescent microscopy
-confocal microscopy
-image analysis
6. Presentations and exam (2 h)
-students work on analyzing results from the practical course and present the results for grading
Oral, presentation of results and discussion thereof, including answering questions of topics covered.
Students work on analyzing results from the practical course and present the results for grading