166.681 Biochemistry
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2023S, SE, 1.0h, 1.0EC


  • Semester hours: 1.0
  • Credits: 1.0
  • Type: SE Seminar
  • Format: Presence

Learning outcomes

After successful completion of the course, students are able to explain the basics of biochemistry, molecular biology, microbiology and bioprocess engineering and to apply them for the upcoming laboratory exercise. These basics include:

  • Microbiology and cell biology: types of microorganisms (pro- and eukaryotes), their occurrence, properties, benefits, dangers, the basic requirements for microbiological work, transgenic organisms and DNA extraction.
  • Molecular biology: Genotyping PCR, agarose electrophoresis, quantitative PCR, DNA sequencing.
  • Cell factories: Recombinant protein production, biotechnological process, production organisms, gene expression, post-translational modifications, protein expression on E. coli, cloning and expression vectors, vector construction, insertion of a DNA fragment into a plasmid, transcriptional regulation.
  • Fermentation: production organisms, risk groups, bacterial expression systems, basic principle of bioreactors, kinetics of growth: batch, fed-batch
  • Protein purification and analysis: Downstreaming processing (DSP), design of DSP, influencing factors, cell separation (centrifugation, filtration), cell disruption, protein purification: chromatography; protein analysis: Protein content, enzyme activity and immunochemical methods.
  • Enzyme kinetics: definition, properties, effect, structure (co-factor, co-enzyme), classification of enzymes; enzymatic reaction, enzyme kinetics (Michaelis Menten equation, Lineweaver Burk diagram and its further developments)

Subject of course

Introduction to the theoretical and practical basics of selected biochemical exercises on microbiology, cell biology, molecular biology, cell factories, fermentation, protein purification and analysis and enzyme kinetics.

Teaching methods

Lecture with the possibility to ask questions, supported by slides provided in TISS and explanations on the blackboard. Furthermore, examples of exercises are calculated with the lecturers.

Mode of examination


Additional information

Sollte aufgrund von Vorgaben der Bundesregierung bzw. der TU Wien die Abhaltung der Lehrveranstaltung und der Prüfung bzw. der Teilleistungen in Präsenz nicht möglich sein, wird in das Online-Format gewechselt. Durch den Wechsel in das Online-Format können sich die angekündigten Termine ändern.

Please consider the plagiarism guidelines of TU Wien when writing your seminar paper: Directive concerning the handling of plagiarism (PDF)



Course dates

Fri08:00 - 10:0010.03.2023 - 26.05.2023GM 5 Praktikum HS- TCH Biochemie
Biochemistry - Single appointments
Fri10.03.202308:00 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri17.03.202308:00 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri24.03.202308:00 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri31.03.202308:00 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri21.04.202308:00 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri28.04.202308:00 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri05.05.202308:00 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri26.05.202308:00 - 10:00GM 5 Praktikum HS- TCH Biochemie

Examination modalities

After the seminar a written test will be held. The test lasts 60 minutes and consists of 12 tasks. These tasks are composed of:

  • 12 questions distributed over the subject areas

A total of 12 points are awarded for the examination; ≥ 10.5 points correspond to "Sehr Gut", ≥ 9 points to "Gut", ≥ 7.5 points to "Befriedigend", ≥ 6 points to "Genügend" and less than 6 points to "Nicht Genügend", which means that the examination must be repeated.


DayTimeDateRoomMode of examinationApplication timeApplication modeExam
Tue16:00 - 18:0023.01.2024Seminarraum BA 02B written04.08.2023 09:00 - 16.01.2024 09:00TISSPrüfung 3 WS23

Course registration

Begin End Deregistration end
01.02.2023 09:00 28.04.2023 09:00


Study CodeObligationSemesterPrecon.Info
033 290 Technical Chemistry Mandatory4. Semester
066 473 Chemical and Process Engineering for Sustainable Production Not specified


No lecture notes are available.

Previous knowledge

Attendance and a positive exam on "Biochemistry 1" is highly recommended.