166.681 Biochemistry
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2022S, SE, 1.0h, 1.0EC

Properties

  • Semester hours: 1.0
  • Credits: 1.0
  • Type: SE Seminar
  • Format: Presence

Learning outcomes

After successful completion of the course, students are able to explain the basics of biochemistry, molecular biology, microbiology and bioprocess engineering and to apply them for the upcoming laboratory exercise. These basics include:

  • Microbiology and cell biology: types of microorganisms (pro- and eukaryotes), their occurrence, properties, benefits, dangers, the basic requirements for microbiological work, transgenic organisms and DNA extraction.
  • Molecular biology: Genotyping PCR, agarose electrophoresis, quantitative PCR, DNA sequencing.
  • Cell factories: Recombinant protein production, biotechnological process, production organisms, gene expression, post-translational modifications, protein expression on E. coli, cloning and expression vectors, vector construction, insertion of a DNA fragment into a plasmid, transcriptional regulation.
  • Fermentation: production organisms, risk groups, bacterial expression systems, basic principle of bioreactors, kinetics of growth: batch, fed-batch
  • Protein purification and analysis: Downstreaming processing (DSP), design of DSP, influencing factors, cell separation (centrifugation, filtration), cell disruption, protein purification: chromatography; protein analysis: Protein content, enzyme activity and immunochemical methods.
  • Enzyme kinetics: definition, properties, effect, structure (co-factor, co-enzyme), classification of enzymes; enzymatic reaction, enzyme kinetics (Michaelis Menten equation, Lineweaver Burk diagram and its further developments)

Subject of course

Introduction to the theoretical and practical basics of selected biochemical exercises on microbiology, cell biology, molecular biology, cell factories, fermentation, protein purification and analysis and enzyme kinetics.

Teaching methods

Lecture with the possibility to ask questions, supported by slides provided in TISS and explanations on the blackboard. Furthermore, examples of exercises are calculated with the lecturers.

Mode of examination

Written

Additional information

Sollte aufgrund von Vorgaben der Bundesregierung bzw. der TU Wien die Abhaltung der Lehrveranstaltung und der Prüfung bzw. der Teilleistungen in Präsenz nicht möglich sein, wird in das Online-Format gewechselt. Durch den Wechsel in das Online-Format können sich die angekündigten Termine ändern.

Please consider the plagiarism guidelines of TU Wien when writing your seminar paper: Directive concerning the handling of plagiarism (PDF)

Lecturers

Institute

Course dates

DayTimeDateLocationDescription
Fri08:30 - 10:0011.03.2022 - 20.05.2022GM 5 Praktikum HS- TCH Biochemie
Biochemistry - Single appointments
DayDateTimeLocationDescription
Fri11.03.202208:30 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri18.03.202208:30 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri25.03.202208:30 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri01.04.202208:30 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri08.04.202208:30 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri29.04.202208:30 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri06.05.202208:30 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri13.05.202208:30 - 10:00GM 5 Praktikum HS- TCH Biochemie
Fri20.05.202208:30 - 10:00GM 5 Praktikum HS- TCH Biochemie

Examination modalities

After the end of the seminar a written examination will be held. The exam lasts 90 minutes and consists of 8 tasks. These tasks are composed of:

  • 8 questions distributed over the subject areas

A total of 8 points are awarded for the examination; ≥ 7 points correspond to "Sehr Gut", ≥ 6 points to "Gut", ≥ 5 points to "Befriedigend", ≥ 4 points to "Genügend" and less than 4 points to "Nicht Genügend", which means that the examination must be repeated.

Course registration

Begin End Deregistration end
02.02.2022 09:00 29.04.2022 09:00

Curricula

Literature

No lecture notes are available.

Previous knowledge

Attendance and a positive exam on "Biochemistry 1" is highly recommended.

Language

German