166.681 Biochemistry
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2021S, SE, 1.0h, 1.0EC
Lecture TubeTUWEL

Properties

  • Semester hours: 1.0
  • Credits: 1.0
  • Type: SE Seminar
  • LectureTube course
  • Format: Distance Learning

Learning outcomes

After successful completion of the course, students are able to explain the basics of biochemistry, molecular biology, microbiology and bioprocess engineering and to apply them for the upcoming laboratory exercise. These basics include:

  • Microbiology and cell biology: types of microorganisms (pro- and eukaryotes), their occurrence, properties, benefits, dangers, the basic requirements for microbiological work, transgenic organisms and DNA extraction.
  • Molecular biology: Genotyping PCR, agarose electrophoresis, quantitative PCR, DNA sequencing.
  • Cell factories: Recombinant protein production, biotechnological process, production organisms, gene expression, post-translational modifications, protein expression on E. coli, cloning and expression vectors, vector construction, insertion of a DNA fragment into a plasmid, transcriptional regulation.
  • Fermentation: production organisms, risk groups, bacterial expression systems, basic principle of bioreactors, kinetics of growth: batch, fed-batch
  • Protein purification and analysis: Downstreaming processing (DSP), design of DSP, influencing factors, cell separation (centrifugation, filtration), cell disruption, protein purification: chromatography; protein analysis: Protein content, enzyme activity and immunochemical methods.
  • Enzyme kinetics: definition, properties, effect, structure (co-factor, co-enzyme), classification of enzymes; enzymatic reaction, enzyme kinetics (Michaelis Menten equation, Lineweaver Burk diagram and its further developments)

Subject of course

Introduction to the theoretical and practical basics of selected biochemical exercises on microbiology, cell biology, molecular biology, cell factories, fermentation, protein purification and analysis and enzyme kinetics.

Teaching methods

Lecture with the possibility to ask questions, supported by slides provided in TISS and explanations on the blackboard. Furthermore, examples of exercises are calculated with the lecturers.

Mode of examination

Written

Additional information

Sollte aufgrund von Vorgaben der Bundesregierung bzw. der TU Wien die Abhaltung der Lehrveranstaltung und der Prüfung bzw. der Teilleistungen in Präsenz wieder möglich sein, wird in das Präsenz-Format gewechselt. Durch den Wechsel in das Präsenz-Format können sich die für die online-Lehrveranstaltung und -prüfung (-teilleistungen) angekündigten Termine ändern. 

Please consider the plagiarism guidelines of TU Wien when writing your seminar paper: Directive concerning the handling of plagiarism (PDF)

Lecturers

Institute

Course dates

DayTimeDateLocationDescription
Fri08:30 - 10:0019.03.2021 - 07.05.2021 online VO via Zoom (LIVE)Biochemie
Biochemistry - Single appointments
DayDateTimeLocationDescription
Fri19.03.202108:30 - 10:00 online VO via ZoomDerntl - Einf Biochemie und MIBI
Fri26.03.202108:30 - 10:00 online VO via ZoomReischer - Einf MOBI
Fri16.04.202108:30 - 10:00 online VO via ZoomSteiger - Klonierung
Fri23.04.202108:30 - 10:00 online VO via ZoomSeiboth - rek. Proteinproduktion
Fri30.04.202108:30 - 10:00 online VO via ZoomPflügl - Fermentation
Fri07.05.202108:30 - 10:00 online VO via ZoomSpadiut - Proteinreinigung und Enzymkinetik

Examination modalities

After the end of the seminar a written examination will be held. The exam lasts 90 minutes and consists of 8 tasks. These tasks are composed of:

  • 8 questions distributed over the subject areas

A total of 8 points are awarded for the examination; ≥ 7 points correspond to "Sehr Gut", ≥ 6 points to "Gut", ≥ 5 points to "Befriedigend", ≥ 4 points to "Genügend" and less than 4 points to "Nicht Genügend", which means that the examination must be repeated.

Exams

DayTimeDateRoomMode of examinationApplication timeApplication modeExam
Fri09:00 - 11:0029.10.2021 Onlinewritten31.08.2021 09:00 - 22.10.2021 09:00TISSTermin WS2
Fri09:00 - 11:0003.12.2021 Onlinewritten31.08.2021 09:00 - 26.11.2021 09:00TISSTermin WS3
Wed11:30 - 13:3019.01.2022 Onlinewritten08.09.2021 09:00 - 12.01.2022 09:00TISSTermin WS4

Course registration

Begin End Deregistration end
01.03.2021 09:00 30.06.2021 09:00

Curricula

Literature

No lecture notes are available.

Previous knowledge

Attendance and a positive exam on "Biochemistry 1" is highly recommended.

Language

German