After successful completion of the course, students are able to grow a microorganism for enzyme production, to purify the produced enzyme and characterize it kinetically. In addition, the students will be able to characterize the microbial production host by molecularbiological methods.
Sterile handling and basic microbiological techniques. Recombinant production of an enzyme in fermentation. Purification and kinetic characterization of the enzyme. Analysis of the microbial production strain by molecuar biology methods (PCR).
The contents of the laboratory course are conveyed by a working instruction and the experiments described therein. A deepening of the teaching content is achieved by the preparation of a laboratory protocol by the students. In addition, open questions are addressed through preparation, results and protocol discussions.
Registration for the courses will take place from 24.08.2020 (8.00) to 27.09.2020 (23.59) via TISS. Please register directly into one of the different groups. For WS 2020/21 the course is limited to 72 participants. Further courses are planned for the summer semester 2021.The prerequisite for registration is the positive completion of the VO Biochemistry I. If the number of registrations exceeds the number of available laboratory places and thus a ranking of the registered students with fulfilled entrance requirements becomes necessary, the following ranking criteria apply:Ranking criterion 1: Positive passing of the exam Mathematik für TCH I (104.084). Date and grade of this exam is NOT a criterion.Ranking criterion 2: Date of the VO Biochemistry I examination (172.695)Ranking criterion 3: Grade of the examination VO Biochemistry I (172,695)Ranking criterion 4: Date of registration for the laboratory exerciseAs it is probably necessary to rank the registered students with fulfilled entrance requirements, the registration will be carried out according to the following procedure:All students who have already completed the Mathematics for Technical Chemistry I AND the VO Biochemistry I can register directly at TISS.All students who have not yet completed Mathematics for Technical Chemistry I, but who have passed the VO Biochemistry I, cannot register directly via TISS. They have to send me their certificate of Biochemistry I by email (bernhard.seiboth@tuwien.ac.at), so that I can make a ranking. Please indicate in the email your desired course + all possible alternative courses. The course allocation for students who have only completed VO Biochemistry 1 will take place from 28.09 on.For all courses there will be a compulsory preliminary meeting. The preliminary meeting will take place online. Detailed information will follow from 28 September.A written test will take place before the courses start. Exact dates for the individual courses will be available in TISS from the end of September. Content is theory and execution of the exercises as stated in the Arbeitsvorschrift. The Arbeitsvorschrift will be published in TISS at the beginning of September.The course takes place in the student laboratory in the BH building on the ground floor. The procedure and details can be found in TISS from September.Please address questions to Dr. Bernhard Seiboth, preferably by email.
Written exam at the beginning of the course. Performance within the lab course. Delivery of the lab protocol.
Course sign in via TISS
The student must have at least 1 of the course(s) completed listed below:
Lecture notes for this course are available. Contents and practical performance is described in the pdf-file, which will be uploaded to TISS by the beginning of october. The theoretical background can be retrieved from the basic lectures "Biochemistry 1", "Biochemistry Proseminar" and "Introduction to Biotechnology and Bioengineering"
A positive exam about "Biochemistry I" is an essential prerequisite for registration to the course. To attend the course the positive passing of a written exam about the content and background of the individual course examples is a prerequiste.
We further strongly recommend also to obtain a positive exam about "Introduction to Biotechnology and Bioengineering" and "Biochemistry Seminar" before this lab.
