166.222 Biochemistry and Biotechnology
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2019W, LU, 3.5h, 3.5EC, to be held in blocked form

Properties

  • Semester hours: 3.5
  • Credits: 3.5
  • Type: LU Laboratory Exercise

Learning outcomes

After successful completion of the course, students are able to grow a microorganism for enzyme production, to purify the produced enzyme and characterize it kinetically. In addition, the students will be able to characterize the microbial production host by molecularbiological methods.

Subject of course

Sterile handling and basic microbiological techniques. Recombinant production of an enzyme in fermentation. Purification and kinetic characterization of the enzyme. Analysis of the microbial production strain by molecuar biology methods (PCR).

Teaching methods

The contents of the laboratory course are conveyed by a working instruction and the experiments described therein. A deepening of the teaching content is achieved by the preparation of a laboratory protocol by the students. In addition, open questions are addressed through preparation, results and protocol discussions.

Mode of examination

Written and oral

Additional information

Registration for the courses starts with 27.08.2018 (08.00) via TISS and ends on the 28.09.2018 (18.00).

Registration for the courses depends on the positive passing of the lecture Biochemistry I. Due to limited resources it may be necessary to rank the number of registered students.

The first criterion for this ranking is the positive passing of the exam Mathematik für TCH I (104.084). The date of the exam and the mark is no criterion.

The second criterion will be the date of the passing of the lecture Biochemistry I (172.695)

The third criterion is the mark of the lecture Biochemistry I (172.695).

The fourth criterion is the registration date for the lab

Students which have passed Mathematik für Technische Chemie I and Biochemie I can register directly via TISS. All students which have not passed the lecture Mathematik für Technische Chemie I but passed the lecture Biochemie I, cannot register directly via TISS. For their registration it is necessary to send me their certificate that they passed Biochemie 1 per email (to: bernhard.seiboth@tuwien.ac.at). Please indicate the course number in which you want to participate and all alternative courses in case this course is overbooked. The final assignment to the lab course will follow end of September.

For all courses a compulsory general meeting will take place on the 1st of October at 8.30 in the Radingerhörsaal.

Before the course a written exam will take place. The eaxcat dates will be announced in September. The content of the exam is the theory and the practical part of the course (course script). The course script will be provided online in TISS by the beginning of September.

Start of the course is in the students lab in the BH building on the ground floor. Details are found in TISS in September.

 For questions please contact Univ. Ass. Dr Bernhard Seiboth preferably per email.

Lecturers

Institute

Course dates

DayTimeDateLocationDescription
Tue08:00 - 10:0001.10.2019GM 2 Radinger Hörsaal Vorbesprechung LU 166.222: Biochemie und Biotechnologie
Fri09:00 - 10:0004.10.2019Seminarraum BH Biosciences Schriftliche Vorbesprechung Biochemie und Biotechnologie
Tue09:00 - 10:0008.10.2019 - 26.11.2019Seminarraum BA 02A Schriftliche Vorbesprechung Biochemie und Biotechnologie
Biochemistry and Biotechnology - Single appointments
DayDateTimeLocationDescription
Tue01.10.201908:00 - 10:00GM 2 Radinger Hörsaal Vorbesprechung LU 166.222: Biochemie und Biotechnologie
Fri04.10.201909:00 - 10:00Seminarraum BH Biosciences Schriftliche Vorbesprechung Biochemie und Biotechnologie
Tue08.10.201909:00 - 10:00Seminarraum BA 02A Schriftliche Vorbesprechung Biochemie und Biotechnologie
Tue15.10.201909:00 - 10:00Seminarraum BA 02A Schriftliche Vorbesprechung Biochemie und Biotechnologie
Tue22.10.201909:00 - 10:00Seminarraum BA 02A Schriftliche Vorbesprechung Biochemie und Biotechnologie
Tue29.10.201909:00 - 10:00Seminarraum BA 02A Schriftliche Vorbesprechung Biochemie und Biotechnologie
Tue05.11.201909:00 - 10:00Seminarraum BA 02A Schriftliche Vorbesprechung Biochemie und Biotechnologie
Tue12.11.201909:00 - 10:00Seminarraum BA 02A Schriftliche Vorbesprechung Biochemie und Biotechnologie
Tue19.11.201909:00 - 10:00Seminarraum BA 02A Schriftliche Vorbesprechung Biochemie und Biotechnologie
Tue26.11.201909:00 - 10:00Seminarraum BA 02A Schriftliche Vorbesprechung Biochemie und Biotechnologie
Course is held blocked

Examination modalities

Written exam at the beginning of the course. Performance within the lab course. Delivery of the lab protocol.

Course registration

Begin End Deregistration end
26.08.2019 08:00 29.09.2019 23:59 29.09.2019 23:59

Registration modalities:

Course sign in via TISS

Precondition

The student must have at least 2 of the course(s) completed listed below:

Group Registration

GroupRegistration FromTo
Kurs A (7.-16.10.2019)26.08.2019 08:0029.09.2019 23:59
Kurs B (14.-23.10.2019)26.08.2019 08:0029.09.2019 23:59
Kurs C (21.-30.10.2019)26.08.2019 00:0829.09.2019 23:59
Kurs D (28.10-06.11.2019)26.08.2019 08:0029.09.2019 23:59
Kurs E (4.-13.11.2019)26.08.2019 08:0029.09.2019 23:59
Kurs F (11.-20.11.2019)26.08.2019 08:0029.09.2019 23:59
Kurs G (18.-27.11.2019)26.08.2019 08:0029.09.2019 23:59
Kurs H (25.11-4.12.2019)26.08.2019 08:0029.09.2019 23:59
Kurs I (2.12-11.12.2019)26.08.2019 08:0029.09.2019 23:59
Kurs J (9.-18.12.2019)26.08.2019 08:0029.09.2019 23:59

Curricula

Study CodeSemesterPrecon.Info
033 290 Technical Chemistry 5. SemesterSTEOP
Course requires the completion of the introductory and orientation phase

Literature

Lecture notes for this course are available. Contents and practical performance is described in the pdf-file, which will be uploaded to TISS by the beginning of october. The theoretical background can be retrieved from the basic lectures "Biochemistry 1", "Biochemistry Proseminar" and "Introduction to Biotechnology and Bioengineering"

Previous knowledge

A positive exam about "Biochemistry I" is an essential prerequisite for registration to the course. To attend the course the positive passing of a written exam about the content and background of the individual course examples is a prerequiste.

We further strongly recommend also to obtain a positive exam about "Introduction to Biotechnology and Bioengineering" and "Biochemistry Seminar" before this lab.

Miscellaneous

  • Attendance Required!

Language

German