After successful completion of the course, students are able to understand the main methods of molecular biology and genome analysis from a theoretical point of view. This will be an important prerequisite for the application of such in laboratories, either as a student in university courses or as researcher at work.

- Structure and properties of the DNA and genomes of pro- and eucaryonts

- Isolation, characterisation and manipulation of genes (gene libraries, screening of gene libraries including special techniques such as the 1-hybrid and 2-hybrid system, etc.

- DNA-sequencing, enzymes for DNA modification, DNA modifying methods

- Detection of gene expression (transcript analysis, reporter systems)

- Insertion of DNA in organisms (transformation, transfection) including plants and mammals

- Genome wide analysis strategies (sequencing of genomes, DNA arrays etc.)

- DNA analytics, genome analysis and insights in selected industrial microorganisms

- Characterization of chromatin and epigenetic structures of genomes

Introduction into the methods of molecular biology and genome analyses and explanation of their application using examples.

Written exam.

Slides for this course are available. download from TISS

Main:

From Genes to Genomes: Concepts and Applications of DNA Technology, Publisher: Whiley- Blackwell, 3rd Edition December 2012, 

ISBN : 978-0-470-68385-9

Accompanying 

Gentechnische Methoden (Labor im Fokus)

Publisher: Spektrum-Akademischer Vlg; Auflage: 2. (2002)

ISBN-13: 978-3827413345

Der Experimentator

Publisher: Spektrum-Akademischer Vlg; Auflage: 4. A. (2003)

ISBN-13: 978-3827414601