164.355 Proteomics
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2022W, VO, 1.5h, 2.0EC
Lecture TubeTUWEL

Properties

  • Semester hours: 1.5
  • Credits: 2.0
  • Type: VO Lecture
  • LectureTube course
  • Format: Distance Learning

Learning outcomes

After successful completion of the course, students are able to identify proteomic questions in basic and applied research and quality control. The learning goal is to know different proteomic strategies and choose the appropriate strategy for diverse questions. Students will be able to design different proteomic experiments and theoretically apply sample preparation, proteomic analyses and bioinformatic analyses. This includes knowledge of protein and peptide separation techniques, such as electrophoresis and liquid chromatography (LC), (tandem) mass spectrometry (MS/MS), ion mobility (IMS) and LC-(IMS)-MS/MS of for identification, characterisation and quantification of proteins and peptides, and functional annotation of proteins and their modifications. Moreover, students will learn to apply statistical tools to proteomic data to calculate protein identification confidence and significant quantitative changes.

Subject of course

Proteomic strategies and methods: definition of research question, different sample preparation methods, identification, quantitation and annotation of proteins, peptides and proteomes, structural characterisation of proteins and their modifications, functional proteomics, high-through-put technologies, (tandem) mass spectrometry, ion mobility, liquid chromatography, bioinformatic tools

Teaching methods

Lecture with practical exercises (e.g. manual MS/MS spectrum interpretation of peptides, usage of webtools)

presentation and discussion of examples of proteomic applications

Mode of examination

Immanent

Lecturers

Institute

Course dates

DayTimeDateLocationDescription
Tue16:00 - 19:0011.10.2022 - 13.12.2022 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09Zoom online Lecture
Tue16:00 - 19:0015.11.2022 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09 (LIVE)Proteomics lecture
Proteomics - Single appointments
DayDateTimeLocationDescription
Tue11.10.202216:00 - 19:00 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09Zoom online Lecture
Tue18.10.202216:00 - 19:00 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09Zoom online Lecture
Tue08.11.202216:00 - 19:00 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09Zoom online Lecture
Tue15.11.202216:00 - 19:00 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09Proteomics lecture
Tue22.11.202216:00 - 19:00 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09Zoom online Lecture
Tue29.11.202216:00 - 19:00 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09Zoom online Lecture
Tue06.12.202216:00 - 19:00 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09Zoom online Lecture
Tue13.12.202216:00 - 19:00 https://tuwien.zoom.us/j/94048263126?pwd=ellpcVVXM1doZmt0M1hRNDVxQURIZz09Zoom online Lecture

Examination modalities

Presentation and discussion of a proteomics application example

Exams

DayTimeDateRoomMode of examinationApplication timeApplication modeExam
Tue16:00 - 19:0006.12.2022Seminarraum Lehar 02 oralno application-Proteomics exam
Tue16:00 - 19:0013.12.2022Seminarraum Lehar 02 oralno application-Proteomics exam

Course registration

Begin End Deregistration end
04.10.2022 14:00 30.10.2022 00:00 30.10.2022 00:00

Curricula

Study CodeSemesterPrecon.Info
066 490 Technical Chemistry

Literature

Copies of the ppt-slides will be available via TISS/TUWEL.

Previous knowledge

Knowledge of protein chemistry, biochemistry and instrumental analytical chemistry is essential.

Miscellaneous

  • Attendance Required!

Language

English