After successful completion of the course, students are able to explain principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques, to identify principles and efficiencies of "high end" mass-spectrometric analyser, to categorise different mass-spectrometers as well as to name typical application examples in the field of biomedical engineering.

• Principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques (desorption Electrospray Ionization (DESI), vacuum matrix Assisted LDI (vMALDI, ESI and nano ESI)
• principles and efficiencies of "high end" mass-spectrometric analyzers: Linear and reflectron time-of-flight analyser, quadrupoles, 2D and 3D ion traps inclusive orbitrap
• Tandem and multi-stage mass spectrometer for collision-induced dissociation
• Imaging and profiling mass spectrometry
• Application of the methods on qualitative (structure-referred) and quantitative questions from the medicine and medical pharmacology/toxicology.

• concept: inverted classroom - self study, meetings for assessment to monitor progress of study
• practice section on mass spectrometers (Summer 2021: according to COVID-19 restrictions)
• slides and videos suitable to achieve the learning objective

• oral examination
• Exam Dates announced and booked via TISS, Zoom Link provided in TUWEL

The attendence of the laboratory course is essential to finish this VU.

Copies of Powerpoint slides will be available via TISS.

E. de Hoffmann and V. Stroobant, Mass spectrometry - principles and applications (3rd edition) Wiley, 2007 (Paperback).

Lecture series Instrumentell Analytical Biochemistry (164.234) and basic concepts of biochemistry/chemistry.