164.235 Biomedical Mass Spectrometry
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2020S, VU, 2.0h, 3.0EC
This course is evaluated following the new mode. Learn more

Course evaluation


  • Semester hours: 2.0
  • Credits: 3.0
  • Type: VU Lecture and Exercise

Learning outcomes

After successful completion of the course, students are able to explain principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques, to identify principles and efficiencies of "high end" mass-spectrometric analyser, to categorise different mass-spectrometers as well as to name typical application examples in the field of biomedical engineering.

Subject of course

  • Principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques (desorption Electrospray Ionization (DESI), vacuum matrix Assisted LDI (vMALDI, ESI and nano ESI)
  • principles and efficiencies of "high end" mass-spectrometric analyzers: Linear and reflectron time-of-flight analyser, quadrupoles, 2D and 3D ion traps inclusive orbitrap
  • Tandem and multi-stage mass spectrometer for collision-induced dissociation
  • Imaging and profiling mass spectrometry
  • Application of the methods on qualitative (structure-referred) and quantitative questions from the medicine and medical pharmacology/toxicology.

Teaching methods

  • lecture
  • practice section on mass spectrometers
  • slides suitable to achieve the learning objective

Mode of examination


Additional information

The first lecture day also determines when the exercise will take place.

Exercise part: details will be announced by E. Pittenauer (registration required by E. Pittenauer via e-mail)



Course dates

Thu17:00 - 18:3012.03.2020Seminarraum BA 02A BMMS-BME

Examination modalities

  • oral examination
  • Appointment after personal agreement

Course registration

Begin End Deregistration end
03.03.2020 00:00 02.07.2020 00:00

Registration modalities:

The attendence of the laboratory course is essential to finish this VU.



Copies of Powerpoint slides will be available via TISS.

E. de Hoffmann and V. Stroobant, Mass spectrometry - principles and applications (3rd edition) Wiley, 2007 (Paperback).

Previous knowledge

Lecture series Instrumentell Analytical Biochemistry (164.234) and basic concepts of biochemistry/chemistry.