164.235 Biomedical Mass Spectrometry
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2020S, VU, 2.0h, 3.0EC


  • Semester hours: 2.0
  • Credits: 3.0
  • Type: VU Lecture and Exercise

Learning outcomes

After successful completion of the course, students are able to explain the function of the most important ionization methods and mass analyzers as well as typical application examples in the field of biomedical engineering.

Subject of course

  • Principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques (desorption Electrospray Ionization (DESI), vacuum matrix Assisted LDI (vMALDI, ESI and nano ESI)
  • principles and efficiencies of "high end" mass-spectrometric analyzers: Linear and reflectron time-of-flight analyser, quadrupoles, 2D and 3D ion traps inclusive orbitrap
  • Tandem and multi-stage mass spectrometer for collision-induced dissociation
  • Imaging and profiling mass spectrometry
  • Application of the methods on qualitative (structure-referred) and quantitative questions from the medicine and medical pharmacology/toxicology.

Teaching methods

  • lecture
  • practice section on mass spectrometers
  • slides suitable to achieve the learning objective

Mode of examination


Additional information

The first lecture day also determines when the exercise will take place.

Exercise part: details will be announced by E. Pittenauer (registration required by E. Pittenauer via e-mail)



Course dates

Thu17:00 - 18:3012.03.2020Seminarraum BA 02A BMMS-BME
Thu17:00 - 18:3019.03.2020 - 25.06.2020Seminarraum BA 02A BMMS BME
Biomedical Mass Spectrometry - Single appointments
Thu12.03.202017:00 - 18:30Seminarraum BA 02A BMMS-BME
Thu19.03.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu26.03.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu02.04.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu23.04.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu30.04.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu07.05.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu14.05.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu28.05.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu04.06.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu18.06.202017:00 - 18:30Seminarraum BA 02A BMMS BME
Thu25.06.202017:00 - 18:30Seminarraum BA 02A BMMS BME

Examination modalities

  • oral examination
  • Appointment after personal agreement

Course registration

Begin End Deregistration end
03.03.2020 00:00 02.07.2020 00:00

Registration modalities:

The attendence of the laboratory course is essential to finish this VU.



Copies of Powerpoint slides will be available via TISS.

E. de Hoffmann and V. Stroobant, Mass spectrometry - principles and applications (3rd edition) Wiley, 2007 (Paperback).

Previous knowledge

Lecture series Instrumentell Analytical Biochemistry (164.234) and basic concepts of biochemistry/chemistry.