Introduction into the mass spectrometric concepts and instrumentation applied in the biomedical field as well as application of these techniques in medicine.

Principles and mechanisms of the gaseous phase ionization techniques and desorption/ionization techniques (desorption Electrospray Ionization (DESI), vacuum matrix Assisted LDI (vMALDI, ESI and nano ESI); principles and efficiencies of "high end" mass-spectrometric analyzers: Linear and reflectron time-of-flight analyser, quadrupoles, 2D and 3D ion traps inclusive orbitrap; Tandem and multi-stage mass spectrometer for collision-induced dissociation; Imaging and profiling mass spectrometry; Application of the methods on qualitative (structure-referred) and quantitative questions from the medicine and medical pharmacology/toxicology.

The start will take place on 14th March 2019 at 5 pm in the seminar room SEM BA 02A and the practical part will be fixed during this meeting.

Lectures every Thursday from 5:00 to 6:30 pm. Location: S

Practical part: Details will be given by E. Pittenauer (registration via Ernst Pittenauer (via e-mail) is required due to limited space)

Oral examination

The attendence of the laboratory course is essential to finish this VU.

Copies of Powerpoint slides will be available via TISS.

E. de Hoffmann and V. Stroobant, Mass spectrometry - principles and applications (3rd edition) Wiley, 2007 (Paperback).

Lecture series Instrumentell Analytical Biochemistry (164.234) and basic concepts of biochemistry/chemistry.