1. Modul: Introduction and Basics (G. Haberhauer) - Introduction to the structure of the lecture - Micro Arrays & Biochips - Definition - Area of Applications - Overview of different array systems - Analytical concepts & technologies - Theoretical limits of the approaches - Short introduction to the following moduls 2. Modul: DNA & Protein Chips - Surface and detection systems (C. Preininger) - surfaces & immobilization o substrates (glass, plastic, metal) and substrate activation o surface functionalization (reactive polymers and particles; coating methods; nanostructuring; 1D (i.E. SAMs) vs. 2D (i.E. PEG) vs. 3D (i.E. hydrogels)) o Immobilization methods (binding principles, differences between DNA and proteins) o Interaction between immobilized biomolecule and surface o Surface requirements with respect to detection system and on-chip assay - detection - optical systems o fluorescence o (electro)chemiluminescence o surface plasmon resonance o labels: indicators, liposomes, nanoparticles, quantum dots 3. Modul: DNA Chips - Marker identification & applications (A.Sessitsch) - Diagnostic chips and requirements; - molecular/phylogenetic markers, - Probe design - Short and long oligonucleotide probes - Diversity arrays; - Spezifity and Sensitivity - Examples of use 4. Modul: Cell Chips (P. Ertl) o what is µTAS, history and overview (from concept to µTAS) o microfluidics for cell cultures (theoretical aspects and components) o cell patterning techniques (microcontact, microfluidic, stencil patterning) o cell sampling, manipulation and treatment (trapping, sorting, lysis, transfection, fusion etc. ) o cell analysis (electrical, optical, magnetic etc.) o single cell analysis (ion channel studies etc.) o cell culture analogues (microphysiometers, neuronal biosensors etc.) o future prospects (integration of nanotechnology)
