164.220 Biochip Technologies in (Bio)Analytical Chemistry
Diese Lehrveranstaltung ist in allen zugeordneten Curricula Teil der STEOP.
Diese Lehrveranstaltung ist in mindestens einem zugeordneten Curriculum Teil der STEOP.

2019S, VO, 2.0h, 3.0EC

Merkmale

  • Semesterwochenstunden: 2.0
  • ECTS: 3.0
  • Typ: VO Vorlesung

Ziele der Lehrveranstaltung

To give an overview of current biochip technolgies, different platforms and possibly applications. In addition we will present new developments in the field of DNA/Protein and Cell Chips.

Inhalt der Lehrveranstaltung

1. Modul: Introduction and Basics (G. Haberhauer) - Introduction to the structure of the lecture - Micro Arrays & Biochips - Definition - Area of Applications - Overview of different array systems - Analytical concepts & technologies - Theoretical limits of the approaches - Short introduction to the following moduls 2. Modul: DNA & Protein Chips - Surface and detection systems (C. Preininger) - surfaces & immobilization o substrates (glass, plastic, metal) and substrate activation o surface functionalization (reactive polymers and particles; coating methods; nanostructuring; 1D (i.E. SAMs) vs. 2D (i.E. PEG) vs. 3D (i.E. hydrogels)) o Immobilization methods (binding principles, differences between DNA and proteins) o Interaction between immobilized biomolecule and surface o Surface requirements with respect to detection system and on-chip assay - detection - optical systems o fluorescence o (electro)chemiluminescence o surface plasmon resonance o labels: indicators, liposomes, nanoparticles, quantum dots 3. Modul: DNA Chips - Marker identification & applications (A.Sessitsch) - Diagnostic chips and requirements; - molecular/phylogenetic markers, - Probe design - Short and long oligonucleotide probes - Diversity arrays; - Spezifity and Sensitivity - Examples of use 4. Modul: Cell Chips (P. Ertl) o what is µTAS, history and overview (from concept to µTAS) o microfluidics for cell cultures (theoretical aspects and components) o cell patterning techniques (microcontact, microfluidic, stencil patterning) o cell sampling, manipulation and treatment (trapping, sorting, lysis, transfection, fusion etc. ) o cell analysis (electrical, optical, magnetic etc.) o single cell analysis (ion channel studies etc.) o cell culture analogues (microphysiometers, neuronal biosensors etc.) o future prospects (integration of nanotechnology)

Vortragende

Institut

LVA Termine

TagZeitDatumOrtBeschreibung
Mi.14:00 - 17:0008.05.2019 - 26.06.2019Sem.R. DB gelb 07 Lecture
Biochip Technologies in (Bio)Analytical Chemistry - Einzeltermine
TagDatumZeitOrtBeschreibung
Mi.08.05.201914:00 - 17:00Sem.R. DB gelb 07 Lecture
Mi.15.05.201914:00 - 17:00Sem.R. DB gelb 07 Lecture
Mi.22.05.201914:00 - 17:00Sem.R. DB gelb 07 Lecture
Mi.29.05.201914:00 - 17:00Sem.R. DB gelb 07 Lecture
Mi.05.06.201914:00 - 17:00Sem.R. DB gelb 07 Lecture
Mi.12.06.201914:00 - 17:00Sem.R. DB gelb 07 Lecture
Mi.19.06.201914:00 - 17:00Sem.R. DB gelb 07 Lecture
Mi.26.06.201914:00 - 17:00Sem.R. DB gelb 07 Lecture

Leistungsnachweis

schriftlich

Prüfungen

TagZeitDatumOrtPrüfungsmodusAnmeldefristAnmeldungPrüfung
Mi. - 02.10.2019schriftlich25.06.2019 00:00 - 01.10.2019 00:00in TISSExam II
Mi. - 23.10.2019schriftlich21.09.2019 14:00 - 16.10.2019 14:00in TISSExam - START 13:00
Mo.09:00 - 11:0006.07.2020Seminarraum BA 02A schriftlich10.09.2019 09:00 - 05.07.2020 22:00in TISSEXAM Biochip

LVA-Anmeldung

Von Bis Abmeldung bis
01.03.2019 00:00 30.05.2019 00:00 30.05.2019 00:00

Curricula

Literatur

Es wird kein Skriptum zur Lehrveranstaltung angeboten.

Sprache

Englisch