164.220 Biochip Technologies in (Bio)Analytical Chemistry
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2018S, VO, 2.0h, 3.0EC

Properties

  • Semester hours: 2.0
  • Credits: 3.0
  • Type: VO Lecture

Aim of course

To give an overview of current biochip technolgies, different platforms and possibly applications. In addition we will present new developments in the field of DNA/Protein and Cell Chips.

Subject of course

1. Modul: Introduction and Basics (G. Haberhauer) - Introduction to the structure of the lecture - Micro Arrays & Biochips - Definition - Area of Applications - Overview of different array systems - Analytical concepts & technologies - Theoretical limits of the approaches - Short introduction to the following moduls 2. Modul: DNA & Protein Chips - Surface and detection systems (C. Preininger) - surfaces & immobilization o substrates (glass, plastic, metal) and substrate activation o surface functionalization (reactive polymers and particles; coating methods; nanostructuring; 1D (i.E. SAMs) vs. 2D (i.E. PEG) vs. 3D (i.E. hydrogels)) o Immobilization methods (binding principles, differences between DNA and proteins) o Interaction between immobilized biomolecule and surface o Surface requirements with respect to detection system and on-chip assay - detection - optical systems o fluorescence o (electro)chemiluminescence o surface plasmon resonance o labels: indicators, liposomes, nanoparticles, quantum dots 3. Modul: DNA Chips - Marker identification & applications (A.Sessitsch) - Diagnostic chips and requirements; - molecular/phylogenetic markers, - Probe design - Short and long oligonucleotide probes - Diversity arrays; - Spezifity and Sensitivity - Examples of use 4. Modul: Cell Chips (P. Ertl) o what is µTAS, history and overview (from concept to µTAS) o microfluidics for cell cultures (theoretical aspects and components) o cell patterning techniques (microcontact, microfluidic, stencil patterning) o cell sampling, manipulation and treatment (trapping, sorting, lysis, transfection, fusion etc. ) o cell analysis (electrical, optical, magnetic etc.) o single cell analysis (ion channel studies etc.) o cell culture analogues (microphysiometers, neuronal biosensors etc.) o future prospects (integration of nanotechnology)

Lecturers

Institute

Course dates

DayTimeDateLocationDescription
Wed14:00 - 17:0009.05.2018 - 27.06.2018Sem.R. DB gelb 07 Lecture
Biochip Technologies in (Bio)Analytical Chemistry - Single appointments
DayDateTimeLocationDescription
Wed09.05.201814:00 - 17:00Sem.R. DB gelb 07 Lecture
Wed16.05.201814:00 - 17:00Sem.R. DB gelb 07 Lecture
Wed23.05.201814:00 - 17:00Sem.R. DB gelb 07 Lecture
Wed30.05.201814:00 - 17:00Sem.R. DB gelb 07 Lecture
Wed06.06.201814:00 - 17:00Sem.R. DB gelb 07 Lecture
Wed13.06.201814:00 - 17:00Sem.R. DB gelb 07 Lecture
Wed20.06.201814:00 - 17:00Sem.R. DB gelb 07 Lecture
Wed27.06.201814:00 - 17:00Sem.R. DB gelb 07 Lecture

Examination modalities

schriftlich

Course registration

Begin End Deregistration end
31.12.2017 00:00 26.03.2018 00:00 26.03.2018 00:00

Curricula

Literature

No lecture notes are available.

Language

English