After successful completion of the course, students are able to modify a protein and change its properties. As part of this exercise, the entire workflow, from determining the protein sequence, the correct selection of primers and the work to be carried out in practice, is taught. The data received are then evaluated and summarized in a log.

In addition, the following topics are dealt with in 4 lecture units:

1) Biocatalysis, 2) Protein engineering, 3) High-throughput screenings, 4) Bioinformatics (e.g. next gene sequencing, PyMol)

The goal of this course is to produce enzyme mutants with improved thermostability and changed substrate specificity. To accomplish this goal we will change amino acids in different positions in the enzyme. The positions are chosen rational by analyzing the 3D structure of the enzyme. Positions can be at the binding region between subunits of the enzyme and by increasing the binding strength between the subunits we hope to increase also the thermostability. Other positions can be in the substrate binding pocket and by altering these positions we surly will alter the substrate binding specificity of the enzyme.

The students are introduced to the course content using Powerpoint presentations and hands-on data processing and evaluation. Specifically, PyMol is used as simulation software for the display of proteins.

!!!! Aufgrund der aktuellen Lage wird zu einem späteren Zeitpunkt entschieden, zu welchen Terminen die VO stattfindet, ob es anhand von Videos die Möglichkeit zur Fernlehre geben wird, oder ob die Lehrveranstaltung für dieses Semester abgesagt werden muss!!!

Proof of performance is provided by assessing the practical work and the submitted written protocol. The results should be described and discussed.

Basic molecular biological working techniques such as sterile working, creating protein gels and PCR knowledge are required.

The laboratory exercise is located in the master's degree plan or beyond.