Microscopy becomes an experience in its application. We will offer two approaches in the practical course:

• A primitive microscope will be built on a breadboard based on the plans of Ernst Abbe; it allows for capturing the basics of image formation, resolution and Fourier optics.
• A modern fluorescence microscope will be used to study mammalian cells below the diffraction limit of conventional light microscopy.

• Setup of an Abbe-microscope, principles of dark-field microscopy and Fourier optics
• Köhler illumination, Differential Interference Contrast (DIC)
• Total Internal Reflection Fluorescence (TIRF) Mikroskopie
• Single molecule tracking
• Superresolution microscopy

Literature will be available.

Literature will be available

 Successful completion of "Microscopy on Biomoleculs, VO" is mandatory for participation in the practical course.