134.997 Microscopy on Biomolecules
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2018W, PR, 2.0h, 2.0EC, to be held in blocked form


  • Semester hours: 2.0
  • Credits: 2.0
  • Type: PR Project

Aim of course

Microscopy becomes an experience in its application. We will offer two approaches in the practical course:

  • A primitive microscope will be built on a breadboard based on the plans of Ernst Abbe; it allows for capturing the basics of image formation, resolution and Fourier optics.
  • A modern fluorescence microscope will be used to study mammalian cells below the diffraction limit of conventional light microscopy.

Subject of course

  • Setup of an Abbe-microscope, principles of dark-field microscopy and Fourier optics
  • Köhler illumination, Differential Interference Contrast (DIC)
  • Total Internal Reflection Fluorescence (TIRF) Mikroskopie
  • Single molecule tracking
  • Superresolution microscopy

Additional information

Literature will be available.





Course registration

Begin End Deregistration end
01.01.2019 12:00


Study CodeObligationSemesterPrecon.Info
066 453 Biomedical Engineering Not specified
ALG For all Students Not specified


Literature will be available

Previous knowledge

 Successful completion of "Microscopy on Biomoleculs, VO" is mandatory for participation in the practical course. 


  • Attendance Required!