134.997 Microscopy on Biomolecules
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2018W, PR, 2.0h, 2.0EC, to be held in blocked form

Properties

  • Semester hours: 2.0
  • Credits: 2.0
  • Type: PR Project

Aim of course

Microscopy becomes an experience in its application. We will offer two approaches in the practical course:

  • A primitive microscope will be built on a breadboard based on the plans of Ernst Abbe; it allows for capturing the basics of image formation, resolution and Fourier optics.
  • A modern fluorescence microscope will be used to study mammalian cells below the diffraction limit of conventional light microscopy.

Subject of course

  • Setup of an Abbe-microscope, principles of dark-field microscopy and Fourier optics
  • Köhler illumination, Differential Interference Contrast (DIC)
  • Total Internal Reflection Fluorescence (TIRF) Mikroskopie
  • Single molecule tracking
  • Superresolution microscopy

Additional information

Literature will be available.

 

 

Lecturers

Institute

Course registration

Begin End Deregistration end
01.01.2019 12:00

Curricula

Literature

Literature will be available

Previous knowledge

 Successful completion of "Microscopy on Biomoleculs, VO" is mandatory for participation in the practical course. 

Miscellaneous

  • Attendance Required!

Language

English