After successful completion of the course, students are able to work independently in a cell culture lab. They have mastered aseptic techniques as well as basic cell culture techniques such as cell passaging, cryopreservation and cell counting.
This course is replaced by 134.232 Mammalian Cell Culture in SS2024.
This course provides an introduction to mammalian cell culture, covering topics such as laboratory setup, aseptic techniques and safety aspects. Students will perform the following techniques on both adherent and suspension cell lines: resuspension of mammalian cells from cold storage, sterile media preparation, passaging and feeding of cells, cryopreservation of cells, microscopic evaluation of confluence and morphology, and cell counting. Further, students will perform metabolic activity assays.
The contents of the laboratory course are conveyed by a working instruction and the exercises described therein. An instructor will show and supervise small groups of students.
Evaluation of the practical lab work; small tasks
Participation in the LU requires a positive completion of the accompanying VO. Registration will be confirmed after completion of the VO.
The student must have at least all of the course(s) completed listed below:
Mammalian Cell Culture, VO
