After successful completion of the course, students are able to work independently in a cell culture lab. They have mastered aseptic techniques as well as basic cell culture techniques such as cell passaging, cryopreservation and cell counting.

This course provides an introduction to mammalian cell culture, covering topics such as laboratory setup, aseptic techniques and safety aspects. Students will perform the following techniques on both adherent and suspension cell lines: resuspension of mammalian cells from cold storage, sterile media preparation, passaging and feeding of cells, cryopreservation of cells, microscopic evaluation of confluence and morphology, and cell counting. Further, students will work with 3D spheroid cultures and perform metabolic activity assays.

The contents of the laboratory course are conveyed by a working instruction and the exercises described therein. An instructor will show and supervise small groups of students.

Positive completion of the accompanying VO is mandatory.

Tentative course date: 15./16.6. 9:00-17:00  Please be aware that this date may change due to a lab relocation scheduled for spring.

! Due to low capacities we can only accomodate up to 10 students !

If you are interested in taking the course, please contact: eva.sevcsik@tuwien.ac.at

Immanent

Mammalian Cell Culture, VO