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134.232 Mammalian Cell Culture
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2022W, LU, 1.0h, 1.0EC, to be held in blocked form


  • Semester hours: 1.0
  • Credits: 1.0
  • Type: LU Laboratory Exercise
  • Format: Presence

Learning outcomes

After successful completion of the course, students are able to work independently in a cell culture lab. They have mastered aseptic techniques as well as basic cell culture techniques such as cell passaging, cryopreservation and cell counting.

Subject of course

This course provides an introduction to mammalian cell culture, covering topics such as laboratory setup, aseptic techniques and safety aspects. Students will perform the following techniques on both adherent and suspension cell lines: resuspension of mammalian cells from cold storage, sterile media preparation, passaging and feeding of cells, cryopreservation of cells, microscopic evaluation of confluence and morphology, and cell counting. Further, students will work with 3D spheroid cultures and perform metabolic activity assays.

Teaching methods

The contents of the laboratory course are conveyed by a working instruction and the exercises described therein. An instructor will show and supervise small groups of students.

Mode of examination


Additional information

Participation in the LU requires a positive completion of the accompanying VO. 

The LU will be held in January 2023 (Jan 12+13; 8.30 am - 4 pm).

A second course will be offered upon demand.




Examination modalities


Course registration

Begin End Deregistration end
03.10.2022 08:00 23.12.2022 23:59

Registration modalities

If you are interested in taking the courser, please send an Email to: eva.sevcsik@tuwien.ac.at


Study CodeObligationSemesterPrecon.Info
066 453 Biomedical Engineering Not specified
066 461 Technical Physics Elective
810 FW Elective Courses - Technical Physics Elective


No lecture notes are available.

Previous knowledge

Mammalian Cell Culture, VO

Preceding courses


  • Attendance Required!