134.231 Mammalian Cell Culture
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2024S, VO, 1.0h, 1.5EC, to be held in blocked form

Course evaluation

Properties

  • Semester hours: 1.0
  • Credits: 1.5
  • Type: VO Lecture
  • Format: Presence

Learning outcomes

After successful completion of the course, students are able to independently work in a cell culture lab as well as plan and conduct experiments with cells. They have gained theoretical knowledge of the principles and techniques in mammalian cell culture. 

Subject of course

  • Cell culture basics (laboratory setup, equipment, aseptic technique, contamination, safety aspects)
  • Commonly used cell lines (adherent and suspension cell lines), primary cell culture
  • Commonly used cell culture media and supplements
  • Basic cell culture techniques (cryopreservation of cells, microscopic evaluation of confluence and morphology, cell counting)
  • Transfection and selection, cell line differentiation, characterization of cells
  • 3D cell culture, spheroids
  • Metabolic activity assays
  • Bioprocessing technologies

Teaching methods

Presentation and discussion of the subjects with the students

Depending on the circumstances, the lecture will be held on site

Mode of examination

Written

Additional information

Lecture on Monday March 18 cancelled due to illness!!

 

Lecturers

Institute

Course dates

DayTimeDateLocationDescription
Mon10:00 - 12:0004.03.2024 - 06.05.2024Seminarraum BA 02A 134.231 VO Grundlagen der Zellkultur
Mammalian Cell Culture - Single appointments
DayDateTimeLocationDescription
Mon04.03.202410:00 - 12:00Seminarraum BA 02A 134.231 VO Grundlagen der Zellkultur
Mon11.03.202410:00 - 12:00Seminarraum BA 02A 134.231 VO Grundlagen der Zellkultur
Mon18.03.202410:00 - 12:00Seminarraum BA 02A 134.231 VO Grundlagen der Zellkultur
Mon08.04.202410:00 - 12:00Seminarraum BA 02A 134.231 VO Grundlagen der Zellkultur
Mon15.04.202410:00 - 12:00Seminarraum BA 02A 134.231 VO Grundlagen der Zellkultur
Mon22.04.202410:00 - 12:00Seminarraum BA 02A 134.231 VO Grundlagen der Zellkultur
Mon29.04.202410:00 - 12:00Seminarraum BA 02A 134.231 VO back up date
Mon06.05.202410:00 - 12:00Seminarraum BA 02A Exam
Course is held blocked

Examination modalities

Written exam

 

Exams

DayTimeDateRoomMode of examinationApplication timeApplication modeExam
Tue11:00 - 12:0019.11.2024Seminarraum BA 02B written01.10.2024 12:00 - 18.11.2024 23:00TISSExam, written
Tue11:00 - 12:0026.11.2024Seminarraum BA 02B written01.10.2024 12:00 - 25.11.2024 20:00TISSExam, written

Course registration

Begin End Deregistration end
23.02.2024 13:00 31.05.2024 23:59

Curricula

Study CodeObligationSemesterPrecon.Info
066 453 Biomedical Engineering Not specified
066 461 Technical Physics Elective
810 FW Elective Courses - Technical Physics Elective

Literature

Previous knowledge

Basic knowledge in cell biology, biochemistry strongly recommended

Language

English