134.231 Mammalian Cell Culture
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2023S, VO, 1.0h, 1.5EC, to be held in blocked form

Properties

  • Semester hours: 1.0
  • Credits: 1.5
  • Type: VO Lecture
  • Format: Presence

Learning outcomes

After successful completion of the course, students are able to independently work in a cell culture lab as well as plan and conduct experiments with cells. They have gained theoretical knowledge of the principles and techniques in mammalian cell culture. 

Subject of course

  • Cell culture basics (laboratory setup, equipment, aseptic technique, contamination, safety aspects)
  • Commonly used cell lines (adherent and suspension cell lines), primary cell culture
  • Commonly used cell culture media and supplements
  • Basic cell culture techniques (cryopreservation of cells, microscopic evaluation of confluence and morphology, cell counting)
  • Transfection and selection, cell line differentiation, characterization of cells
  • 3D cell culture, spheroids
  • Metabolic activity assays
  • Bioprocessing technologies

Teaching methods

Presentation and discussion of the subjects with the students

Depending on the circumstances, the lecture will be held on site

Mode of examination

Written

Additional information

Lecture dates: 

 BA Seminar room 08B

13.3.2023 10:00-12:00

20.3.2023 10:00-12:00

27.3.2023 10:15-12:00

17.4.2023 10:15-12:00

BA Seminar room 10B

24.4 14:00-16:00

28.4. 14:00-16:00

 

Lecturers

Institute

Course dates

DayTimeDateLocationDescription
Mon10:00 - 12:0013.03.2023 - 17.04.2023Seminarraum BA 08B - MB Grundlagen der Zellkultur VO
Mon14:00 - 16:0024.04.2023Seminarraum BA 10B - MB Grundlagen der Zellkultur VO
Fri14:00 - 16:0028.04.2023Seminarraum BA 10B - MB Grundlagen der Zellkultur VO
Mammalian Cell Culture - Single appointments
DayDateTimeLocationDescription
Mon13.03.202310:00 - 12:00Seminarraum BA 08B - MB Grundlagen der Zellkultur VO
Mon20.03.202310:00 - 12:00Seminarraum BA 08B - MB Grundlagen der Zellkultur VO
Mon27.03.202310:00 - 12:00Seminarraum BA 08B - MB Grundlagen der Zellkultur VO
Mon17.04.202310:00 - 12:00Seminarraum BA 08B - MB Grundlagen der Zellkultur VO
Mon24.04.202314:00 - 16:00Seminarraum BA 10B - MB Grundlagen der Zellkultur VO
Fri28.04.202314:00 - 16:00Seminarraum BA 10B - MB Grundlagen der Zellkultur VO
Course is held blocked

Examination modalities

Written exam

 

Exams

DayTimeDateRoomMode of examinationApplication timeApplication modeExam
Wed12:00 - 13:0022.05.2024Seminarraum BA 08A - MB written30.04.2024 12:50 - 21.05.2024 23:59TISSExam Mammalian cell culture

Course registration

Begin End Deregistration end
24.02.2023 13:00 02.06.2023 23:59

Curricula

Study CodeObligationSemesterPrecon.Info
066 453 Biomedical Engineering Not specified
066 461 Technical Physics Elective
810 FW Elective Courses - Technical Physics Elective

Literature

Previous knowledge

Basic knowledge in cell biology, biochemistry strongly recommended

Language

English