134.231 Mammalian Cell Culture
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2023S, VO, 1.0h, 1.5EC, to be held in blocked form


  • Semester hours: 1.0
  • Credits: 1.5
  • Type: VO Lecture
  • Format: Presence

Learning outcomes

After successful completion of the course, students are able to independently work in a cell culture lab as well as plan and conduct experiments with cells. They have gained theoretical knowledge of the principles and techniques in mammalian cell culture. 

Subject of course

  • Cell culture basics (laboratory setup, equipment, aseptic technique, contamination, safety aspects)
  • Commonly used cell lines (adherent and suspension cell lines), primary cell culture
  • Commonly used cell culture media and supplements
  • Basic cell culture techniques (cryopreservation of cells, microscopic evaluation of confluence and morphology, cell counting)
  • Transfection and selection, cell line differentiation, characterization of cells
  • 3D cell culture, spheroids
  • Metabolic activity assays
  • Bioprocessing technologies

Teaching methods

Presentation and discussion of the subjects with the students

Depending on the circumstances, the lecture will be held in hybrid mode

Mode of examination


Additional information

Lecture dates: 

Di 26.4. 10:00-12:00

Fr 29.4. 10:00-12:00

Di 3.5. 10:00-12:00

Fr 6.5. 10:00-12:00

The Zoom link for lecture 1-6 is:


Meeting ID: 978 2320 3911
Password: mb3JW5Eh


Mo 9.5. 13:00-15:00

Fr 13.5. 10:00-12:00



May 20 and June 1st, 2022
Place: Zoom: https://tuwien.zoom.us/j/97823203911?pwd=T2hSTkNIL2ptSWNnRlJldTM3VUdMdz09

Please read the pdf describing the exam modalities in the "Unterlagen (Documents)" section.



Examination modalities

Written exam



DayTimeDateRoomMode of examinationApplication timeApplication modeExam
Tue10:00 - 11:0013.12.2022Seminarraum Lehar 01 written03.10.2022 08:00 - 12.12.2022 23:59TISSGrundlagen der Zellkultur Prüfung
Tue10:00 - 11:0020.12.2022Seminarraum Lehar 01 written03.10.2022 08:00 - 19.12.2022 23:59TISSGrundlagen der Zellkutur Prüfung

Course registration

Begin End Deregistration end
14.03.2023 10:00 02.06.2023 23:59