134.231 Mammalian Cell Culture
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2022W, VO, 1.0h, 1.5EC, to be held in blocked form

Properties

  • Semester hours: 1.0
  • Credits: 1.5
  • Type: VO Lecture
  • Format: Presence

Learning outcomes

After successful completion of the course, students are able to independently work in a cell culture lab as well as plan and conduct experiments with cells. They have gained theoretical knowledge of the principles and techniques in mammalian cell culture. 

Subject of course

  • Cell culture basics (laboratory setup, equipment, aseptic technique, contamination, safety aspects)
  • Commonly used cell lines (adherent and suspension cell lines), primary cell culture
  • Commonly used cell culture media and supplements
  • Basic cell culture techniques (cryopreservation of cells, microscopic evaluation of confluence and morphology, cell counting)
  • Transfection and selection, cell line differentiation, characterization of cells
  • 3D cell culture, spheroids
  • Metabolic activity assays
  • Bioprocessing technologies

Teaching methods

Presentation and discussion of the subjects with the students

Mode of examination

Written

Lecturers

Institute

Course dates

DayTimeDateLocationDescription
Tue10:00 - 12:0011.10.2022 - 08.11.2022GM 7 Kleiner Schiffbau VO Mammalian cell culture
Tue10:00 - 12:0022.11.2022 - 29.11.2022Seminarraum BA 10B - MB VO Mammalian Cell Culture
Mammalian Cell Culture - Single appointments
DayDateTimeLocationDescription
Tue11.10.202210:00 - 12:00GM 7 Kleiner Schiffbau VO Mammalian cell culture
Tue18.10.202210:00 - 12:00GM 7 Kleiner Schiffbau VO Mammalian cell culture
Tue25.10.202210:00 - 12:00GM 7 Kleiner Schiffbau VO Mammalian cell culture
Tue08.11.202210:00 - 12:00GM 7 Kleiner Schiffbau VO Mammalian cell culture
Tue22.11.202210:00 - 12:00Seminarraum BA 10B - MB VO Mammalian Cell Culture
Tue29.11.202210:00 - 12:00Seminarraum BA 10B - MB VO Mammalian Cell Culture
Course is held blocked

Examination modalities

Written exam

 

Exams

DayTimeDateRoomMode of examinationApplication timeApplication modeExam
Wed12:00 - 13:0022.05.2024Seminarraum BA 08A - MB written30.04.2024 12:50 - 21.05.2024 23:59TISSExam Mammalian cell culture

Course registration

Begin End Deregistration end
22.08.2022 12:00

Curricula

Study CodeObligationSemesterPrecon.Info
066 453 Biomedical Engineering Not specified
066 461 Technical Physics Elective
810 FW Elective Courses - Technical Physics Elective

Literature

No lecture notes are available.

Previous knowledge

Knowledge in cell biology/biochemistry is recommended.

Language

English