134.220 Superresolution microscopy with single molecules
This course is in all assigned curricula part of the STEOP.
This course is in at least 1 assigned curriculum part of the STEOP.

2019W, LU, 2.0h, 3.0EC

Properties

  • Semester hours: 2.0
  • Credits: 3.0
  • Type: LU Laboratory Exercise

Learning outcomes

After successful completion of the course, students are able to comprehend the sujects presented in the lecture and to draw conclusions from them as well as to actively communicate the contents presented during the lecture.

Subject of course

In the last years, microscopy concepts have been developed which allow for optical resolution below the classical diffraction limit. This practical course will introduce one of these methods, single molecule switching microscopy (acronyms are PALM, STORM, dSTORM, fPALM): single molecules are stochastically switched on and off, and the positions of all detected molecules are determined to an accuracy of ~20nm by mathematical fitting. If requested students can bring and characterize their own samples; alternatively, fluorescently labeled cells will be used as examples.

Teaching methods

After successful completion of the course, students are able to comprehend the sujects presented in the lecture and to draw conclusions from them as well as to actively communicate the contents presented during the lecture.

Mode of examination

Immanent

Lecturers

Institute

Course dates

DayTimeDateLocationDescription
Thu15:00 - 16:0003.10.2019FH Hörsaal 5 - TPH Vorbesprechung SCHÜTZ

Examination modalities

After successful completion of the course, students are able to comprehend the sujects presented in the lecture and to draw conclusions from them as well as to actively communicate the contents presented during the lecture.

Course registration

Not necessary

Curricula

Literature

No lecture notes are available.

Language

if required in English